Tannin and Lignin Method Data

-By Method 5550


Lignin is a plant constituent that often is discharged as a waste during the manufacture of paper pulp. Another plant constituent, tannin, may enter the water supply through the process of vegetable matter degradation or through the wastes of the tanning industry. Tannin also is applied in the so-called internal treatment of boiler waters, where it reduces scale formation by causing the production of a more easily handled sludge.

Both lignin and tannin contain aromatic hydroxyl groups that react with Folin phenol reagent (tungstophosphoric and molybdophosphoric acids) to form a blue color suitable for estimation of concentrations up to at least 9 mg/L. However, the reaction is not specific for lignin or tannin, nor for compounds containing aromatic hydroxyl groups, inasmuch as many other reducing materials, both organic and inorganic, respond similarly.

Method Data

Method 5550
Hold Time
PreservationNo Preservation
Sample Volume250mL HDPE bottle
Collection MethodGrab sampling
Method RefernceSM 5550

Analyte List


AnalyteFormulaCAS Number
Tannin and Lignin


* The typical detection limits and analytes are displayed for this particular method. Please contact Specialty Analytical if other analytes or detection limits are required in addition to the 200.8 Analyte List.

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